ln18 glioma cells  (ATCC)

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 1. The expression of adiponectin receptor in the LN18 glioma cell line.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Expressing

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 2. Effect of adiponectin on LN18 glioma cell proliferation. (a) Dose-dependent relationship of adiponectin on the proliferation of LN18 glioma cells; (b) Time-dependent relationship of adiponectin on the proliferation of LN18 glioma cells. Compared with the control group, *P < 0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 4. Adiponectin regulates the expression of apoptosis-related pro teins in LN18 cells. Compared with the control group, *P<0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Expressing, Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 5. Effect of adiponectin on apoptosis of LN18 glioma cells. (a) Control group; (b) Adiponectin 0.5 μg/mL; (c) Adiponectin 1.0 μg/ mL; (d) Adiponectin 3.0 μg/mL.Compared with the control group, *P<0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 6. Adiponectin regulates the expression of LN18 cell cycle-rela ted proteins. Compared with the control group, *P<0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Expressing, Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 7. Effect of adiponectin on LN18 cell proliferation. (a) Control group, (b) Adiponectin 3.0 μg/mL. Compared with the control group, *P<0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 8. Effect of adiponectin on the expression of autophagy-related proteins in LN18 cells. Compared with the control group, *P<0.05.

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Expressing, Control

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: Regulation and molecular mechanism of adiponectin on the proliferation, apoptosis, autophagy, and chemosensitivity of LN18 Glioma cell line.

doi: 10.14715/cmb/2024.70.6.27

Figure Lengend Snippet: Fig. 9. Adiponectin enhances the inhibitory effect of TMZ on the pro liferation rate of the LN18 cell line. Compared with the control group and excipient group, *P<0.05; compared with TMZ 1.0 mM group,

Article Snippet: Gliomas cell lines LN18, U87MG, and U251 cells were obtained from the American Type Culture Collection (ATCC), thawed from early-passage stocks kept in liquid nitrogen vapor as required, and cultured according to the supplier’s instructions.

Techniques: Control

Journal: Cancer medicine

Article Title: Biomarkers of peripheral blood neutrophil extracellular traps in the diagnosis and progression of malignant tumors.

doi: 10.1002/cam4.6935

Figure Lengend Snippet: FIGURE 1 PMA and glioma cells stimulated neutrophils to release NETs. (A) Neutrophils were stimulated with PMA for 1, 2, 3, and 4 h, respectively, and then stained with Sytox Green (green) and Hoechst 33342 (blue) to observe NETs (scale: 50 μm). (B) Quantitative results of fluorescent staining of the area of PMA-stimulated neutrophils releasing NETs at different time points (ns = not statistically significant, **p < 0.01, ***p < 0.001). (C) PMA stimulation of neutrophils for 4 h, and immunofluorescence staining of both citH3 (green) and DNA (DAPI, blue) was performed to visualize NETs, the white arrows indicate NETs (scale bar: 20 μm). (D) Fluorescence-stained images of LN18 CM stimulated neutrophils for 4 h to release NETs (scale bar: 50 μm). (E) Quantitative results of fluorescence staining of the area of LN18 CM-stimulated neutrophils releasing NETs at 4 h (****p < 0.0001). (F) Immunofluorescence staining images of LN18 CM-stimulated neutrophils releasing NETs at 4 h, the white arrows indicate NETs (scale bar: 20 μm). Error bars in B and E represent SD.

Article Snippet: The human glioma cell line LN18 (American Type Culture Collection, USA) was cultured in DMEM medium (Gibco, USA) containing 5% fetal bovine serum and 1% penicillin– streptomycin.

Techniques: Staining, Immunofluorescence, Fluorescence